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	Provedor de dados BJMBR (3) Autor Carneiro,C.R.W. (3) Leão,S.C. (1) Lima,E.N.P. (1) Lopes,J.D. (1) Matsui,T. (1) Melati,I. (1) Moraes,D.W. (1) Prado,I.B. (1) Solimene,A.C.C. (1) Mais... Palavra-chave Carcinoembryonic antigen (2) CEA (1) Colorectal carcinoma (1) Colorectal carcinoma cell line (1) Desmoglein (1) Fibronectin (1) Gene expression (1) Immunoscintigraphy (1) Laminin (1) Metastasis (1) Monoclonal antibody (1) Mycobacterium tuberculosis (1) Phospholipase C (1) Promoters (1) Technetium-99m (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Ano 2001 (1) 2000 (1) 1999 (1) País Brazil (3) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Evaluation of an anti-carcinoembryonic monoclonal antibody suitable for immunoscintigraphy BJMBR Moraes,D.W.; Lima,E.N.P.; Prado,I.B.; Carneiro,C.R.W.. An anti-carcinoembryonic antigen (CEA) monoclonal antibody (mAb 6D1.1) was evaluated in vitro and in vivo to determine its suitability as a tracer for immunoscintigraphy of colorectal carcinomas. Determination of mAb affinity for CEA showed a constant of association of 0.63 ± 0.11 x 109 M-1. Binding of technetium-99m (99mTc)-6D1.1, labeled by a direct method, to human cultured lineages was highly specific. Binding to only CEA-positive LS-174T cells resulted in a saturable curve inhibited by pre-incubation with unlabeled mAb. No binding at all was observed for the human lineages MeWo (melanoma) or ZR75-30 (breast carcinoma), neither of them expressing CEA cells. Intravenous injection of 99mTc-6D1.1 into nude mice xenografted with human LS-174T tumors... Tipo: Info:eu-repo/semantics/article Palavras-chave: Monoclonal antibody; Carcinoembryonic antigen; Colorectal carcinoma; Immunoscintigraphy; Technetium-99m. Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000800006 Evidence for the expression of native Mycobacterium tuberculosis phospholipase C: recognition by immune sera and detection of promoter activity BJMBR Matsui,T.; Carneiro,C.R.W.; Leão,S.C.. The genome of Mycobacterium tuberculosis H37Rv contains three contiguous genes (plc-a, plc-b and plc-c) which are similar to the Pseudomonas aeruginosa phospholipase C (PLC) genes. Expression of mycobacterial PLC-a and PLC-b in E. coli and M. smegmatis has been reported, whereas expression of the native proteins in M. tuberculosis H37Rv has not been demonstrated. The objective of the present study was to demonstrate that native PLC-a is expressed in M. tuberculosis H37Rv. Sera from mice immunized with recombinant PLC-a expressed in E. coli were used in immunoblots to evaluate PLC-a expression. The immune serum recognized a 49-kDa protein in immunoblots against M. tuberculosis extracts. No bands were visible in M. tuberculosis culture supernatants or... Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycobacterium tuberculosis; Phospholipase C; Gene expression; Promoters. Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001100003 Functional differences between two morphologically distinct cell subpopulations within a human colorectal carcinoma cell line BJMBR Solimene,A.C.C.; Carneiro,C.R.W.; Melati,I.; Lopes,J.D.. The LISP-I human colorectal adenocarcinoma cell line was isolated from a hepatic metastasis at the Ludwig Institute, São Paulo, SP, Brazil. The objective of the present study was to isolate morphologically different subpopulations within the LISP-I cell line, and characterize some of their behavioral aspects such as adhesion to and migration towards extracellular matrix components, expression of intercellular adhesion molecules and tumorigenicity in vitro. Once isolated, the subpopulations were submitted to adhesion and migration assays on laminin and fibronectin (crucial proteins to invasion and metastasis), as well as to anchorage-independent growth. Two morphologically different subpopulations were isolated: LISP-A10 and LISP-E11. LISP-A10 presents a... Tipo: Info:eu-repo/semantics/article Palavras-chave: Carcinoembryonic antigen; CEA; Colorectal carcinoma cell line; Laminin; Fibronectin; Desmoglein; Metastasis. Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000500014 Registros recuperados: 3 Primeira ... 1 ... Última

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